Document Type

Article

Publication Version

Published Version

Publication Date

2014

Journal or Book Title

ACS Nano

Volume

8

Issue

9

First Page

9097

Last Page

9106

DOI

10.1021/nn502551y

Abstract

Biomineralization proteins are widely used as templating agents in biomimetic synthesis of a variety of organic-inorganic nanostructures. However, the role of the protein in controlling the nucleation and growth of biomimetic particles is not well understood, because the mechanism of the bioinspired reaction is often deduced from ex situ analysis of the resultant nanoscale mineral phase. Here we report the direct visualization of biomimetic iron oxide nanoparticle nucleation mediated by an acidic bacterial recombinant protein, Mms6, during an in situ reaction induced by the controlled addition of sodium hydroxide to solution-phase Mms6 protein micelles incubated with ferric chloride. Using in situ liquid cell scanning transmission electron microscopy we observe the liquid iron prenucleation phase and nascent amorphous nanoparticles forming preferentially on the surface of protein micelles. Our results provide insight into the early steps of protein-mediated biomimetic nucleation of iron oxide and point to the importance of an extended protein surface during nanoparticle formation.

Comments

Reprinted (adapted) with permission from ACS Nano 8 (2014): 9097, doi: 10.1021/nn502551y. Copyright 2014 American Chemical Society.

Copyright Owner

American Chemical society

Language

en

File Format

application/pdf

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