Document Type
Article
Publication Date
2015
Journal or Book Title
International Journal of Nanomedicine
Volume
10
First Page
229
Last Page
243
DOI
10.2147/IJN.S72264
Abstract
H5N1 avian influenza is a significant global concern with the potential to become the next pandemic threat. Recombinant subunit vaccines are an attractive alternative for pandemic vaccines compared to traditional vaccine technologies. In particular, polyanhydride nanoparticles encapsulating subunit proteins have been shown to enhance humoral and cell-mediated immunity and provide protection upon lethal challenge. In this work, a recombinant H5 hemagglutinin trimer (H53) was produced and encapsulated into polyanhydride nanoparticles. The studies performed indicated that the recombinant H53 antigen was a robust immunogen. Immunizing mice with H53 encapsulated into polyanhydride nanoparticles induced high neutralizing antibody titers and enhanced CD4+ T cell recall responses in mice. Finally, the H53-based polyanhydride nanovaccine induced protective immunity against a low-pathogenic H5N1 viral challenge. Informatics analyses indicated that mice receiving the nanovaccine formulations and subsequently challenged with virus were similar to naïve mice that were not challenged. The current studies provide a basis to further exploit the advantages of polyanhydride nanovaccines in pandemic scenarios.
Rights
This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License. The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. Permissions beyond the scope of the License are administered by Dove Medical Press Limited. Information on how to request permission may be found at: http://www.dovepress.com/permissions.php
Copyright Owner
Ross et al
Copyright Date
2015
Language
en
File Format
application/pdf
Recommended Citation
Ross, Kathleen Alaine; Loyd, Hyelee Park; Wu, Wuwei; Huntimer, Lucas Mark; Ahmed, Shaheen; Sambol, Anthony; Broderick, Scott; Flickinger, Zachary; Rajan, Krishna; Bronich, Tatiana; Mallapragada, Surya K.; Wannemuehler, Michael J.; Carpenter, Susan Long; and Narasimhan, Balaji, "Hemagglutinin-based polyanhydride nanovaccines against H5N1 influenza elicit protective virus neutralizing titers and cell-mediated immunity" (2015). Chemical and Biological Engineering Publications. 262.
https://lib.dr.iastate.edu/cbe_pubs/262
Included in
Biological Engineering Commons, Chemical Engineering Commons, Materials Science and Engineering Commons, Pathology Commons, Pharmacy and Pharmaceutical Sciences Commons, Veterinary Microbiology and Immunobiology Commons
Comments
This article is from International Journal of Nanomedicine 10 (2015): 229, doi: 10.2147/IJN.S72264. Posted with permission.