Document Type
Article
Publication Version
Published Version
Publication Date
2-2015
Journal or Book Title
Langmuir
Volume
31
First Page
2818
Last Page
2825
DOI
10.1021/la5044377
Abstract
Magnetotactic bacteria that produce magnetic nanocrystals of uniform size and well-defined morphologies have inspired the use of biomineralization protein Mms6 to promote formation of uniform magnetic nanocrystals in vitro. Small angle X-ray scattering (SAXS) studies in physiological solutions reveal that Mms6 forms compact globular threedimensional (3D) micelles (approximately 10 nm in diameter) that are, to a large extent, independent of concentration. In the presence of iron ions in the solutions, the general micellar morphology is preserved, however, with associations among micelles that are induced by iron ions. Compared with Mms6, the m2Mms6 mutant (with the sequence of hydroxyl/carboxyl containing residues in the C-terminal domain shuffled) exhibits subtle morphological changes in the presence of iron ions in solutions. The analysis of the SAXS data is consistent with a hierarchical core−corona micellar structure similar to that found in amphiphilic polymers. The addition of ferric and ferrous iron ions to the protein solution induces morphological changes in the micellar structure by transforming the 3D micelles into objects of reduced dimensionality of 2, with fractal-like characteristics (including Gaussian-chain-like) or, alternatively, platelet-like structures.
Copyright Owner
American Chemical society
Copyright Date
2015
Language
en
File Format
application/pdf
Recommended Citation
Zhang, Honghu; Liu, Xunpei; Feng, Shuren; Wang, Wenjie; Schmidt-Rohr, Klaus; Akinc, Mufit; Nilsen-Hamilton, Marit; Vaknin, David; and Mallapragada, Surya K., "Morphological Transformations in the Magnetite Biomineralizing Protein Mms6 in Iron Solutions: A Small-Angle X‑ray Scattering Study" (2015). Chemical and Biological Engineering Publications. 263.
https://lib.dr.iastate.edu/cbe_pubs/263
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Chemical Engineering Commons, Materials Science and Engineering Commons, Physics Commons
Comments
Reprinted (adapted) with permission from Langmuir 31 (2015): 2818, doi: 10.1021/la5044377. Copyright 2015 American Chemical Society.