Chemical and Biological Engineering, Ames Laboratory, NSF Engineering Research Center for Biorenewable Chemicals
Research Focus Area
Renewable Energy, Biorenewables
Journal or Book Title
Angewandte Chemie International Edition
Biorefineries aim to convert biomass to a spectrum of products ranging from biofuels to specialty chemicals. To achieve economically sustainable conversion it is crucial to streamline the catalytic and downstream processing steps. Here we report a route that integrates bio- and chemical catalysis to convert glucose into bio-based unsaturated nylon 6,6. An engineered strain of Saccharomyces cerevisiae, with the highest reported muconic acid titer of 559.5 mg L-1 in yeast, was used as the initial biocatalyst to convert glucose into muconic acid. Without any separation, muconic acid was further electrocatalytically hydrogenated to 3-hexenedioic acid with 94% yield, despite the presence of all the biogenic impurities. Bio-based unsaturated nylon 6,6 (unsaturated polyamide 6,6) was finally obtained by polymerization of 3-hexenedioic acid with hexamethylenediamine, demonstrating the integrated design of bio-based polyamides from glucose.
Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
Suastegui, Miguel; Matthiesen, John E.; Carraher, Jack M.; Hernández, Nacú; Rodriguez Quiroz, Natalia; Okerlund, Adam; Cochran, Eric W.; Shao, Zengyi; and Tessonnier, Jean-Philippe, "Combining Metabolic Engineering and Electrocatalysis: Application to the Production of Polyamides from Sugar" (2016). Chemical and Biological Engineering Publications. 293.