Chemical and Biological Engineering, Center for Crops Utilization Research
Research Focus Area
Journal or Book Title
Environmental Progress & Sustainable Energy
If biosurfactants are to achieve their promise in environmental oil-spill remediation, their toxicity to marine life must be assessed. A killifish larvae assay is commonly used as a measure of toxicity but is difficult and nonlinear in response. Red blood cell (RBC) hemolysis has also been the basis for assays of some surfactant levels. Here we present a modified sheep RBC suspension assay and compare its response to that of the fish assay for surfactin and its genetically modified variant fatty-acyl-glutamate (FA-Glu). This is the first report of hemolytic activity as a property of FA-Glu. The method's potential for screening for toxicity against marine organisms is demonstrated.
American Institute of Chemical Engineers
Colonna, William J.; Marti, Mustafa E.; Nyman, John A.; Green, Chris; and Glatz, Charles, "Hemolysis as a rapid screening technique for assessing the toxicity of native surfactin and a genetically engineered derivative" (2017). Chemical and Biological Engineering Publications. 306.