Campus Units
Chemical and Biological Engineering, Center for Crops Utilization Research
Document Type
Article
Research Focus Area
Biorenewables
Publication Version
Accepted Manuscript
Publication Date
3-2017
Journal or Book Title
Environmental Progress & Sustainable Energy
Volume
36
Issue
2
First Page
505
Last Page
510
DOI
10.1002/ep.12444
Abstract
If biosurfactants are to achieve their promise in environmental oil-spill remediation, their toxicity to marine life must be assessed. A killifish larvae assay is commonly used as a measure of toxicity but is difficult and nonlinear in response. Red blood cell (RBC) hemolysis has also been the basis for assays of some surfactant levels. Here we present a modified sheep RBC suspension assay and compare its response to that of the fish assay for surfactin and its genetically modified variant fatty-acyl-glutamate (FA-Glu). This is the first report of hemolytic activity as a property of FA-Glu. The method's potential for screening for toxicity against marine organisms is demonstrated.
Copyright Owner
American Institute of Chemical Engineers
Copyright Date
2016
Language
en
File Format
application/pdf
Recommended Citation
Colonna, William J.; Marti, Mustafa E.; Nyman, John A.; Green, Chris; and Glatz, Charles, "Hemolysis as a rapid screening technique for assessing the toxicity of native surfactin and a genetically engineered derivative" (2017). Chemical and Biological Engineering Publications. 306.
https://lib.dr.iastate.edu/cbe_pubs/306
Included in
Biological Engineering Commons, Bioresource and Agricultural Engineering Commons, Chemical Engineering Commons
Comments
This is the peer reviewed version of the following article: Colonna, William J., Mustafa E. Martı, John A. Nyman, Chris Green, and Charles E. Glatz. "Hemolysis as a rapid screening technique for assessing the toxicity of native surfactin and a genetically engineered derivative." Environmental Progress & Sustainable Energy 36, no. 2 (2017): 505-510, which has been published in final form at 10.1002/ep.12444. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.