Campus Units

Chemical and Biological Engineering, Food Science and Human Nutrition, Center for Crops Utilization Research

Document Type

Article

Publication Version

Submitted Manuscript

Publication Date

3-15-2015

Journal or Book Title

Biochemical Engineering Journal

Volume

95

First Page

48

Last Page

55

DOI

10.1016/j.bej.2014.11.011

Abstract

Fatty-acyl-glutamate (FA-Glu), a surfactin variant has been successfully produced using a genetically modified strain of Bacillus subtilis grown on glucose. However, yields with soybean hulls (SBH) replacing glucose were lower. This work was undertaken to reduce the yield loss when using SBH as the carbon source and to evaluate two other soy by-products, namely fiber and skim from aqueous oil extraction as alternative carbon and nitrogen sources.

Fermentation of soybean hulls, fibers and skim at various concentrations produced lower FA-Glu titers compared to S-7 medium. Neither increasing their amount nor supplementing with glucose increased the FA-Glu titer, suggesting the presence of an inhibitor in these feedstocks. By using a mixture of polysaccharide-degrading enzymes, over 65% of SBH solids were converted to soluble carbohydrates. FA-Glu titers obtained from SBH hydrolysates containing residual hull solids were still low; however, with the removal of the solids, cell growth improved and FA-Glu yield was 60% higher than with glucose. Thus, this low-cost material can be converted to a substrate for production of FA-Glu biosurfactant. Unmodified fiber and skim components of aqueous oil extraction were not beneficial.

Comments

This is a manuscript of an article from Marti, Mustafa E., William J. Colonna, Gabriel Reznik, Michelle Pynn, Kevin Jarrell, Buddhi Lamsal, and Charles E. Glatz. "Production of fatty-acyl-glutamate biosurfactant by Bacillus subtilis on soybean co-products." Biochemical Engineering Journal 95 (2015): 48-55. doi: 10.1016/j.bej.2014.11.011. Posted with permission.

Copyright Owner

Elsevier B.V.

Language

en

File Format

application/pdf

Published Version

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