Campus Units

Chemical and Biological Engineering

Document Type

Article

Research Focus Area

Health Care Technology and Biomedical Engineering, Advanced and Nanostructured Materials

Publication Version

Published Version

Publication Date

2015

Journal or Book Title

RSC Advances

Volume

5

Issue

54

First Page

43515

Last Page

43527

DOI

10.1039/C5RA06252G

Abstract

In this study, temperature and pH responsive cationic and amphiphilic pentablock copolymers, which consist of the temperature responsive triblock Pluronic F127 sandwiched between pH responsive PDEAEM (poly(2-diethylaminoethyl methacrylate)) end blocks, were used for the first time in the development of polyplex and gold nanoparticle (AuNP) based multicomponent siRNA delivery systems (MCSs). Copolymers in both systems protected siRNA from external effects, provided cell entry and endosomal escape. The thermoreversible micellization of the hydrophobic PPO block facilitated the cellular entry while the PDEAEM blocks enhanced the endosomal escape through protonated tertiary amine groups by pH buffering. The synergistic advantages of the different blocks showed an enhanced effect in the MCSs due to attachment and surface configuration reasons. The siRNA transfection efficiency of MCSs against luciferase expressing SKOV3 cells was 15% higher than both the polyplexes alone and the commercial siRNA transfection agent Lipofectamine RNAiMax at the same applied dose, without any toxicity. The results indicated that the multicomponent systems based on the responsive cationic pentablock copolymers and gold nanoparticles have promising potential as an efficient siRNA delivery vector for future applications.

Comments

This article is published as Uz, Metin, Surya K. Mallapragada, and Sacide Alsoy Altinkaya. "Responsive pentablock copolymers for siRNA delivery." RSC Advances 5, no. 54 (2015): 43515-43527. 10.1039/C5RA06252G. Posted with permission.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.

Copyright Owner

Royal Society of Chemistry

Language

en

File Format

application/pdf

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