Campus Units

Chemical and Biological Engineering

Document Type

Book Chapter

Research Focus Area

Biorenewables, Catalysis and Reaction Engineering

Publication Version

Published Version

Publication Date

2019

Journal or Book Title

Bioprocessing Technology for Production of Biopharmaceuticals and Bioproducts

First Page

241

Last Page

265

DOI

10.1002/9781119378341.ch7

Abstract

Microbial production of biorenewable fuels and chemicals is often limited by inhibition of the biocatalyst, either by increasing concentrations of the product compound or by contaminant compounds in the biomass‐derived sugars. This inhibition can interfere with economically viable production. Here we discuss typical mechanisms of inhibition and methods for improving biocatalyst robustness. Inhibition often takes the form of inhibition of enzyme activity, depletion of cofactor pools, and membrane damage; methods are discussed for mitigating each of these types of inhibition. Various evolutionary schemes have been developed and implemented on a variety of inhibitory compounds, including butanol, acetic acid, furfural, and ethanol. Reverse engineering of these improved strains can provide insight into new metabolic engineering strategies.

Comments

This is a chapter from Royce, Liam and Laura R. Jarboe. "Metabolic Engineering for Biocatalyst Robustness to Organic Inhibitors." In Bioprocessing Technology for Production of Biopharmaceuticals and Bioproducts, First Edition. Edited by Claire Komives and Weichang Zhou. Hoboken, NJ: John Wiley & Sons, Inc., 2019: 241-265. DOI: 10.1002/9781119378341.ch7. Posted with permission.

Copyright Owner

John Wiley & Sons, Inc.

Language

en

File Format

application/pdf

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