Chemical and Biological Engineering, Kinesiology, Veterinary Microbiology and Preventive Medicine, Veterinary Diagnostic and Production Animal Medicine, Neuroscience, Nanovaccine Institute, Materials Science and Engineering, Ames Laboratory
Research Focus Area
Health Care Technology and Biomedical Engineering, Advanced and Nanostructured Materials
Journal or Book Title
Immunosenescence poses a formidable challenge in designing effective influenza vaccines for aging populations. While approved vaccines against influenza viruses exist, their efficacy in older adults is significantly decreased due to the diminished capabilities of innate and adaptive immune responses. In this work, the ability of a combination nanovaccine containing both recombinant hemagglutinin and nucleoprotein to provide protection against seasonal influenza virus infection was examined in young and aged mice. Vaccine formulations combining two nanoadjuvants, polyanhydride nanoparticles and pentablock copolymer micelles, were shown to enhance protection against challenge compared to each adjuvant alone in young mice. Nanoparticles were shown to enhance in vitro activation of dendritic cells isolated from aged mice, while both nanoadjuvants did not induce proinflammatory cytokine secretion which may be detrimental in aged individuals. In addition, the combination nanovaccine platform was shown to induce demonstrable antibody titers in both young and aged mice that correlated with the maintenance of body weight post-challenge. Collectively, these data demonstrate that the combination nanovaccine platform is a promising technology for influenza vaccines for older adults.
The Royal Society of Chemistry
Ross, Kathleen A.; Senapati, Sujata; Alley, Jessica; Darling, Ross; Goodman, Jonathan; Jefferson, Matthew; Uz, Metin; Guo, Baoqing; Yoon, Kyoung-Jin; Verhoeven, David; Kohut, Marian; Mallapragada, Surya; Wannemuehler, Michael; and Narasimhan, Balaji, "Single dose combination nanovaccine provides protection against influenza A virus in young and aged mice" (2019). Chemical and Biological Engineering Publications. 355.