Campus Units

Chemical and Biological Engineering, Genetics, Development and Cell Biology, Materials Science and Engineering, Neuroscience, Ames Laboratory

Document Type

Article

Research Focus Area

Health Care Technology and Biomedical Engineering

Publication Version

Submitted Manuscript

Publication Date

2-1-2019

Journal or Book Title

Industrial and Engineering Chemistry Research

DOI

10.1021/acs.iecr.8b05537

Abstract

In this study, gelatin and graphene-based nerve regeneration conduits/scaffolds possessing tailored 3D microstructures and mechanical properties were fabricated using 3D printing. The effect of 3D conduit microstructure and mechanical properties along with the applied electrical stimuli on mesenchymal stem cell (MSCs) behavior and transdifferentiation into Schwann cell (SC)-like phenotypes were investigated. The results indicated that the gelatin conduits/scaffolds had favorable 3D microstructural and mechanical properties for MSC attachment and growth. Immunocytochemistry results demonstrated that the application of electrical stimuli through the conductive graphene within the gelatin-based 3D microstructure had a profound effect on the differentiation of MSCs to SC-like phenotypes and their paracrine activity. 80% of the cells exhibited SC marker staining, and the cells showed significantly enhanced nerve growth factor (NGF) secretion. These results suggest that the electrical stimuli applied within the 3D gelatin matrix enables enhanced differentiation and paracrine activity compared to transdifferentiation procedures involving electrical stimuli applied on 2D substrates and chemical stimuli applied in 3D gelatin scaffolds, leading to promising nerve regeneration strategies.

Comments

This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Industrial & Engineering Chemistry Research, copyright © American Chemical Society after peer review. To access the final edited and published work see DOI: 10.1021/acs.iecr.8b05537.

Copyright Owner

American Chemical Society

Language

en

File Format

application/pdf

Published Version

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