Campus Units

Chemical and Biological Engineering, Nanovaccine Institute

Document Type

Article

Research Focus Area

Advanced and Nanostructured Materials, Health Care Technology and Biomedical Engineering

Publication Version

Accepted Manuscript

Publication Date

7-15-2019

Journal or Book Title

Nanomedicine: Nanotechnology, Biology and Medicine

First Page

102055

DOI

10.1016/j.nano.2019.102055

Abstract

The primary objective of this study was to enhance the antitumor efficacy of a model cancer vaccine through co-delivery of pentaerythritol lipid A (PELA), an immunological adjuvant, and a model tumor antigen, ovalbumin (OVA), separately loaded into polyanhydride particles (PA). In vitro experiments showed that encapsulation of PELA into PA (PA-PELA) significantly enhanced its stimulatory capacity on dendritic cells as evidenced by increased levels of the cell surface costimulatory molecules, CD80/CD86. In vivo experiments showed that PA-PELA, in combination with OVA-loaded PA (PA-OVA), significantly expanded the OVA-specific CD8+ T lymphocyte population compared to PA-OVA alone. Furthermore, serum OVA-specific antibody titers of mice vaccinated with PA-OVA/PA-PELA displayed a significantly stronger shift toward a Th1-biased immune response compared to PA-OVA alone, as evidenced by the substantially higher IgG2C:IgG1 ratios achieved by the former. Analysis of E.G7-OVA tumor growth curves showed that mice vaccinated with PA-OVA/PA-PELA had the slowest average tumor growth rate.

Comments

This is a manuscript of an article published as Wafa, Emad I., Sean M. Geary, Kathleen A. Ross, Jonathan T. Goodman, Balaji Narasimhan, and Aliasger K. Salem. "Pentaerythritol-based lipid A bolsters the antitumor efficacy of a polyanhydride particle-based cancer vaccine." Nanomedicine: Nanotechnology, Biology and Medicine (2019): 102055. DOI: 10.1016/j.nano.2019.102055. Posted with permission.

Copyright Owner

Elsevier Inc.

Language

en

File Format

application/pdf

Available for download on Wednesday, July 15, 2020

Published Version

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