Campus Units

Chemical and Biological Engineering, Microbiology

Document Type

Article

Research Focus Area

Catalysis and Reaction Engineering, Renewable Energy

Publication Version

Published Version

Publication Date

2-3-2020

Journal or Book Title

AMB Express

Volume

10

First Page

28

DOI

10.1186/s13568-020-0965-4

Abstract

Mechanocatalysis is a promising method for depolymerization of lignocellulosic biomass. Microbial utilization of the resulting oligosaccharides is one potential route of adding value to the depolymerized biomass. However, it is unclear how readily these oligosaccharides are utilized by standard cell factories. Here, we investigate utilization of cellulose subjected to mechanocatalytic depolymerization, using ethanologenic Escherichia coli as a model fermentation organism. The mechanocatalytic oligosaccharides supported ethanol titers similar to those observed when glucose was provided at comparable concentrations. Tracking of the various oligomers, using maltose (alpha-1,4) and cellobiose (beta-1,4) oligomers as representative standards of the orientation, but not linkage, of the glycosidic bond, suggests that the malto-like-oligomers are more readily utilized than cello-like-oligomers, consistent with poor growth with cellotetraose or cellopentaose as sole carbon source. Thus, mechanocatalytic oligosaccharides are a promising substrate for cell factories, and microbial utilization of these sugars could possibly be improved by addressing utilization of cello-like oligomers.

Comments

This article is published as Jin, Tao, Mats Käldström, Adriana Benavides, Marcelo D. Kaufman Rechulski, and Laura R. Jarboe. "Utilization of mechanocatalytic oligosaccharides by ethanologenic Escherichia coli as a model microbial cell factory." AMB Express 10 (2020): 28. DOI: 10.1186/s13568-020-0965-4. Posted with permission.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Copyright Owner

The Author(s)

Language

en

File Format

application/pdf

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