Campus Units
Chemical and Biological Engineering, Microbiology
Document Type
Article
Research Focus Area
Catalysis and Reaction Engineering, Renewable Energy
Publication Version
Published Version
Publication Date
2-3-2020
Journal or Book Title
AMB Express
Volume
10
First Page
28
DOI
10.1186/s13568-020-0965-4
Abstract
Mechanocatalysis is a promising method for depolymerization of lignocellulosic biomass. Microbial utilization of the resulting oligosaccharides is one potential route of adding value to the depolymerized biomass. However, it is unclear how readily these oligosaccharides are utilized by standard cell factories. Here, we investigate utilization of cellulose subjected to mechanocatalytic depolymerization, using ethanologenic Escherichia coli as a model fermentation organism. The mechanocatalytic oligosaccharides supported ethanol titers similar to those observed when glucose was provided at comparable concentrations. Tracking of the various oligomers, using maltose (alpha-1,4) and cellobiose (beta-1,4) oligomers as representative standards of the orientation, but not linkage, of the glycosidic bond, suggests that the malto-like-oligomers are more readily utilized than cello-like-oligomers, consistent with poor growth with cellotetraose or cellopentaose as sole carbon source. Thus, mechanocatalytic oligosaccharides are a promising substrate for cell factories, and microbial utilization of these sugars could possibly be improved by addressing utilization of cello-like oligomers.
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright Owner
The Author(s)
Copyright Date
2020
Language
en
File Format
application/pdf
Recommended Citation
Jin, Tao; Käldström, Mats; Benavides, Adriana; Kaufman Rechulski, Marcelo D.; and Jarboe, Laura R., "Utilization of mechanocatalytic oligosaccharides by ethanologenic Escherichia coli as a model microbial cell factory" (2020). Chemical and Biological Engineering Publications. 407.
https://lib.dr.iastate.edu/cbe_pubs/407
Included in
Catalysis and Reaction Engineering Commons, Microbiology Commons, Oil, Gas, and Energy Commons
Comments
This article is published as Jin, Tao, Mats Käldström, Adriana Benavides, Marcelo D. Kaufman Rechulski, and Laura R. Jarboe. "Utilization of mechanocatalytic oligosaccharides by ethanologenic Escherichia coli as a model microbial cell factory." AMB Express 10 (2020): 28. DOI: 10.1186/s13568-020-0965-4. Posted with permission.