Campus Units
Chemical and Biological Engineering
Document Type
Article
Research Focus Area
Advanced and Nanostructured Materials
Publication Version
Published Version
Publication Date
1-28-2020
Journal or Book Title
ACS Nano
Volume
14
Issue
1
First Page
651
Last Page
663
DOI
10.1021/acsnano.9b07326
Abstract
The overexpression of immunomarker programmed cell death protein 1 (PD-1) and engagement of PD-1 to its ligand, PD-L1, are involved in the functional impairment of cluster of differentiation 8+ (CD8+) T cells, contributing to cancer progression. However, heterogeneities in PD-L1 expression and variabilities in biopsy-based assays render current approaches inaccurate in predicting PD-L1 status. Therefore, PD-L1 screening alone is not predictive of patient response to treatment, which motivates us to simultaneously detect multiple immunomarkers engaged in immune modulation. Here, we have developed multimodal probes, immunoactive gold nanostars (IGNs), that accurately detect PD-L1+ tumor cells and CD8+ T cells simultaneously in vivo, surpassing the limitations of current immunoimaging techniques. IGNs integrate the whole-body imaging of positron emission tomography with high sensitivity and multiplexing of Raman spectroscopy, enabling the dynamic tracking of both immunomarkers. IGNs also monitor response to immunotherapies in mice treated with combinatorial PD-L1 and CD137 agonists and distinguish responders from those nonresponsive to treatment. Our results showed a multifunctional nanoscale probe with capabilities that cannot be achieved with either modality alone, allowing multiplexed immunologic tumor profiling critical for predicting early response to immunotherapies.
Rights
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Language
en
File Format
application/pdf
Recommended Citation
Ou, Yu-Chuan; Wen, Xiaona; Johnson, Christopher A.; Shae, Daniel; Ayala, Oscar D.; Webb, Joseph A.; Lin, Eugene C.; DeLapp, Rossane C.; Boyd, Kelli L.; Richmond, Ann; Mahadevan-Jansen, Anita; Rafat, Marjan; Wilson, John T.; Balko, Justin M.; Tantawy, Mohammed N.; Vilgelm, Anna E.; and Bardhan, Rizia, "Multimodal Multiplexed Immunoimaging with Nanostars to Detect Multiple Immunomarkers and Monitor Response to Immunotherapies" (2020). Chemical and Biological Engineering Publications. 408.
https://lib.dr.iastate.edu/cbe_pubs/408
Correction
Included in
Biochemical and Biomolecular Engineering Commons, Medical Immunology Commons, Nanoscience and Nanotechnology Commons
Comments
This article is published as Ou, Yu-Chuan, Xiaona Wen, Christopher A. Johnson, Daniel Shae, Oscar D. Ayala, Joseph A. Webb, Eugene C. Lin, et al. "Multimodal Multiplexed Immunoimaging with Nanostars to Detect Multiple Immunomarkers and Monitor Response to Immunotherapies." ACS Nano 14, no. 1 (2020). DOI: 10.1021/acsnano.9b07326.