Campus Units

Chemical and Biological Engineering, Nanovaccine Institute

Document Type

Article

Research Focus Area

Advanced and Nanostructured Materials, Health Care Technology and Biomedical Engineering

Publication Version

Accepted Manuscript

Publication Date

5-19-2020

Journal or Book Title

Carbohydrate Polymers

First Page

116434

DOI

10.1016/j.carbpol.2020.116434

Abstract

Poor induction of mucosal immunity in the intestines by current Salmonella vaccines is a challenge to the poultry industry. We prepared and tested an oral deliverable Salmonella subunit vaccine containing immunogenic outer membrane proteins (OMPs) and flagellin (F) protein loaded and F-protein surface coated chitosan nanoparticles (CS NPs) (OMPs-F-CS NPs). The OMPs-F-CS NPs had mean particle size distribution of 514 nm, high positive charge and spherical in shape. In vitro and in vivo studies revealed the F-protein surface coated CS NPs were specifically targeted to chicken immune cells. The OMPs-F-CS NPs treatment of chicken immune cells upregulated TLRs, and Th1 and Th2 cytokines mRNA expression. Oral delivery of OMPs-F-CS NPs in birds enhanced the specific systemic IgG and mucosal IgA antibody responses as well as reduced the challenge Salmonella load in the intestines. Thus, user friendly oral deliverable chitosan-based Salmonella vaccine for poultry is a viable alternative to current vaccines.

Comments

This article is published as Renu, Sankar, Yi Han, Santosh Dhakal, Yashavanth S. Lakshmanappa, Shristi Ghimire, Ninoshkaly Feliciano-Ruiz, Sujata Senapati, Balaji Narasimhan, Ramesh Selvaraj, and Gourapura J. Renukaradhya. "Chitosan-adjuvanted Salmonella subunit nanoparticle vaccine for poultry delivered through drinking water and feed." Carbohydrate Polymers (2020): 116434. DOI: 10.1016/j.carbpol.2020.116434. Posted with permission.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Copyright Owner

The Author(s)

Language

en

File Format

application/pdf

Published Version

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