Campus Units

Chemical and Biological Engineering, Nanovaccine Institute

Document Type


Research Focus Area

Advanced and Nanostructured Materials, Health Care Technology and Biomedical Engineering

Publication Version

Accepted Manuscript

Publication Date


Journal or Book Title

Carbohydrate Polymers

First Page





Poor induction of mucosal immunity in the intestines by current Salmonella vaccines is a challenge to the poultry industry. We prepared and tested an oral deliverable Salmonella subunit vaccine containing immunogenic outer membrane proteins (OMPs) and flagellin (F) protein loaded and F-protein surface coated chitosan nanoparticles (CS NPs) (OMPs-F-CS NPs). The OMPs-F-CS NPs had mean particle size distribution of 514 nm, high positive charge and spherical in shape. In vitro and in vivo studies revealed the F-protein surface coated CS NPs were specifically targeted to chicken immune cells. The OMPs-F-CS NPs treatment of chicken immune cells upregulated TLRs, and Th1 and Th2 cytokines mRNA expression. Oral delivery of OMPs-F-CS NPs in birds enhanced the specific systemic IgG and mucosal IgA antibody responses as well as reduced the challenge Salmonella load in the intestines. Thus, user friendly oral deliverable chitosan-based Salmonella vaccine for poultry is a viable alternative to current vaccines.


This article is published as Renu, Sankar, Yi Han, Santosh Dhakal, Yashavanth S. Lakshmanappa, Shristi Ghimire, Ninoshkaly Feliciano-Ruiz, Sujata Senapati, Balaji Narasimhan, Ramesh Selvaraj, and Gourapura J. Renukaradhya. "Chitosan-adjuvanted Salmonella subunit nanoparticle vaccine for poultry delivered through drinking water and feed." Carbohydrate Polymers (2020): 116434. DOI: 10.1016/j.carbpol.2020.116434. Posted with permission.

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The Author(s)



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Published Version