Document Type

Article

Research Focus Area

Biorenewables

Publication Date

5-3-2006

Journal or Book Title

Journal of Agricultural and Food Chemistry

Volume

54

Issue

9

First Page

3181

Last Page

3185

DOI

10.1021/jf052016k

Abstract

A colorimetric method was established to determine the activity of recombinant lipase in extracts from transgenic corn seed. The system was an oil-in-water emulsion that was stabilized by a surfactant to accommodate the organic phase substrate and aqueous phase enzyme. The lipase activity was measured by monitoring the release of nitrophenol at 346 nm from the substrate, 4-nitrophenyl butyrate. Emulsions prepared with various surfactant types and concentrations were tested. For each surfactant, the measured activity was greatest when the surfactant concentration was close to the critical micelle concentration, consistent with the changing trend of oil droplet size as a function of surfactant concentration. The optimal system, with 0.01% (w/w) Tween 80, demonstrated good reproducibility, high sensitivity, robustness, and a linear response to lipase concentration.

Comments

Reprinted with permission from Journal of Agricultural and Food Chemistry 54 (2006): 3181–3185, doi:10.1021/jf052016k, Copyright 2006 American Chemical Society.

Copyright Owner

American Chemical Society

Language

en

File Format

application/pdf

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