Rapid Identification of Staphylococcus aureus and Methicillin Resistance by Flow Cytometry Using a Peptide Nucleic Acid Probe

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2011-09-01
Authors
Shrestha, Nabin
Scalera, Nikole
Wilson, Deborah
Brehm-Stecher, Byron
Procop, Gary
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Food Science and Human Nutrition
Abstract

A total of 56 Staphylococcus aureus isolates incubated for 2 h in the presence or absence of oxacillin were analyzed by flow cytometry after labeling with an S. aureus-specific peptide nucleic acid (PNA) probe. Two defined ratios, the paired signal count ratio (PSCR) and the gate signal count ratio (GSCR), differentiated methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA) with sensitivities of 100% each and specificities of 96% and 100%, respectively.

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This article is from Journal of Clinical Microbiology 49, no. 9 (September 2011): 3383–3385, doi:10.1128/JCM.01098-11.

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Sat Jan 01 00:00:00 UTC 2011
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