Correlating blood immune parameters and a CCT7 genetic variant with the shedding of Salmonella enterica serovar Typhimurium in swine
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The Department of Animal Science originally concerned itself with teaching the selection, breeding, feeding and care of livestock. Today it continues this study of the symbiotic relationship between animals and humans, with practical focuses on agribusiness, science, and animal management.
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The Department of Animal Husbandry was established in 1898. The name of the department was changed to the Department of Animal Science in 1962. The Department of Poultry Science was merged into the department in 1971.
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- Department of Animal Husbandry (1898–1962)
- College of Agricultural and Life Sciences (parent college)
- Department of Poultry Science (merged with, 1971)
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Abstract
The porcine response to Salmonella infection is critical for control of Salmonella fecal shedding and the establishment of Salmonella carrier status. In this study, 40 crossbred pigs were intranasally inoculated with Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) and monitored for Salmonella fecal shedding and blood immune parameters at 2, 7, 14 and 20 days post-inoculation (dpi). Using a multivariate permutation test, a positive correlation was observed between Salmonella Typhimurium shedding levels at 2 and 7 dpi and serum interferon-gamma (IFNγ) levels at 2 dpi (p < 0.05), with Salmonella being shed in greater numbers from animals with higher IFNγ levels. A positive correlation was also observed between IFNγ levels and the number of banded neutrophils (2 dpi), circulating neutrophils (7 and 14 dpi), monocytes (7 dpi), and white blood cells (WBCs) (7, 14 and 20 dpi). We have further performed association studies on these immune response parameters as well as shedding status of the Salmonella-infected pigs with a single nucleotide polymorphism (SNP) in the porcine gene CCT7, previously shown by our group to be transcriptionally up-regulated in swine experimentally inoculated with Salmonella Typhimurium. Our analyses with the 40 pigs suggest a positive association (p = 0.0012) of SNP genotype A/G at position AK240296.c1153G > A of the CCT7 gene with Salmonella shedding at 7 dpi compared to the G/G homozygote genotype. Linking specific genes and genetic polymorphisms with the porcine immune response to Salmonella infection and shedding may identify potential markers for carrier pigs as well as targets for disease diagnosis, intervention and prevention.
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This article is published as Uthe, J. J., Y. Wang, L. Qu, D. Nettleton, C. K. Tuggle, and S. M. D. Bearson. "Correlating blood immune parameters and a CCT7 genetic variant with the shedding of Salmonella enterica serovar Typhimurium in swine." Veterinary microbiology 135, no. 3-4 (2009): 384-388. doi: 10.1016/j.vetmic.2008.09.074.