Source and Description of Primers. Primers for the Cocaine- and Amphetamine-Regulated Transcript ( CART) gene were designed from homologous regions of published human and rat CART sequences (Gen- Bank accession nos. U20325 and U10071, respectively). The primers were used to amplify a 468-bp fragment of the porcine CART gene spanning exons 1 and 2. Porcine CART sequences were sequenced from animals of the Meishan and Hampshire breeds (GenBank accession nos. AFO81918 and AFO81919, respectively) and showed 96% exonic nucleotide identity to the corresponding human CART sequence. Additionally, pig-specific primers were designed for use of the pig/rodent somatic cell hybrids to physically map the CART gene.