Rapid Communication: TaqI Restriction Fragment Length Polymorphism at the Porcine Bone Morphogenetic Protein 6 (BMPG) Locus
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The Department of Animal Science originally concerned itself with teaching the selection, breeding, feeding and care of livestock. Today it continues this study of the symbiotic relationship between animals and humans, with practical focuses on agribusiness, science, and animal management.
History
The Department of Animal Husbandry was established in 1898. The name of the department was changed to the Department of Animal Science in 1962. The Department of Poultry Science was merged into the department in 1971.
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- Department of Animal Husbandry (1898–1962)
- College of Agricultural and Life Sciences (parent college)
- Department of Poultry Science (merged with, 1971)
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Abstract
Source and Description of Probe. A 1.1-kb human cDNA clone for bone morphogenetic protein 6 (BMP6) was excised from the clone U2-7 (Celeste et al., 1990). Method of Detection. Genomic DNA was isolated from whole blood and digested with TuqI. Fragments were separated by agarose gel electrophoresis and akaline vacuum-transferred to charged nylon membranes. Hybridizations were at 65°C for 16 to 20 h (10% dextran sulfate, .5 M NaC1, .05 M sodium phosphate, pH 6.5, 5x Denhardt's, .5% SDS, 100 pg/ mL sonicated, denatured salmon sperm DNA). Final washes were at 60°C in .7x SSC, 3% SDS for 15 to 20 min
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This is an article from Journal of Animal Science 73 (1995): 312, doi:/1995.731312x. Posted with permission.