The Pacific White Shrimp (Litopenaeus vannamei) is important to the aquaculture industry and as a food source, but only limited genome information exists. The demand for specific shrimp broodstocks with high disease resistance and growth rate is increasing so the construction of a genetic map with easily usable genetic markers is a high priority. Using all the available public genomics information, a largescale discovery effort for single nucleotide polymorphism (SNP) genetic markers has produced the identification of 1,576 SNPs in 545 unique contigs. The genotyping work will be performed on the standardized Sequenom TM platform on a ready-to-use shrimp resource family, which will be used to develop a shrimp genetic map, as well as facilitate the mapping of quantitative trait locus (QTL) for growth rate and disease resistance.