Sooty blotch and flyspeck fungi(SBFS) infect the cuticles of the apples resulting in dark blemishes on the surface. The number of species identified in the SBFS has increased with the help of molecular techniques. The following report summarizes the progress of confirming the identity of additional SBFS species using a modified Koch’s postulates. Molecular techniques of DNA extraction and amplification were applied to fungi isolated from signs on apples.