Salmonella is a bacterium that can infect chickens, contaminating meat and eggs, resulting in production losses and illness in consumers. This study was designed to analyze the metabolic effects of Salmonella infection in broilers. Livers were harvested from broilers at ten days post-infection with Salmonella. RNA was isolated and then transformed into cDNA. Genes were analyzed on a global level using microarray technology. Among the differentially expressed genes with known function, gene expression of 29 out of 30 genes was lower in Salmonella-challenged birds than non-challenged birds. Genes associated with the cell cycle along with DNA replication, recombination, and repair functions were the most significantly differentially expressed genes. Salmonella infection resulted in reduced expression of genes involved in cell death, protein recycling, blood pressure increase, cell proliferation, extracellular kinase activation and suppression of immune signaling. The revealed pathways help to determine the systemic effects of Salmonella infection, which may lead to genome-directed methods of disease control in poultry.