Evaluation of Volatile Metabolites Emitted In-Vivo from Cold-Hardy Grapes during Ripening Using SPME and GC-MS: A Proof-of-Concept
Date
Authors
Major Professor
Advisor
Committee Member
Journal Title
Journal ISSN
Volume Title
Publisher
Authors
Research Projects
Organizational Units
The Department of Civil, Construction, and Environmental Engineering seeks to apply knowledge of the laws, forces, and materials of nature to the construction, planning, design, and maintenance of public and private facilities. The Civil Engineering option focuses on transportation systems, bridges, roads, water systems and dams, pollution control, etc. The Construction Engineering option focuses on construction project engineering, design, management, etc.
History
The Department of Civil Engineering was founded in 1889. In 1987 it changed its name to the Department of Civil and Construction Engineering. In 2003 it changed its name to the Department of Civil, Construction and Environmental Engineering.
Dates of Existence
1889-present
Historical Names
- Department of Civil Engineering (1889-1987)
- Department of Civil and Construction Engineering (1987-2003)
- Department of Civil, Construction and Environmental Engineering (2003–present)
Related Units
- College of Engineering (parent college)
Since 1905, the Department of Agricultural Engineering, now the Department of Agricultural and Biosystems Engineering (ABE), has been a leader in providing engineering solutions to agricultural problems in the United States and the world. The department’s original mission was to mechanize agriculture. That mission has evolved to encompass a global view of the entire food production system–the wise management of natural resources in the production, processing, storage, handling, and use of food fiber and other biological products.
History
In 1905 Agricultural Engineering was recognized as a subdivision of the Department of Agronomy, and in 1907 it was recognized as a unique department. It was renamed the Department of Agricultural and Biosystems Engineering in 1990. The department merged with the Department of Industrial Education and Technology in 2004.
Dates of Existence
1905–present
Historical Names
- Department of Agricultural Engineering (1907–1990)
Related Units
- College of Agriculture and Life Sciences (parent college)
- College of Engineering (parent college)
- Department of Industrial Education and Technology, (merged, 2004)
Journal Issue
Is Version Of
Versions
Series
Department
Abstract
In this research, we propose a novel concept for a non-destructive evaluation of volatiles emitted from ripening grapes using solid-phase microextraction (SPME). This concept is novel to both the traditional vinifera grapes and the cold-hardy cultivars. Our sample models are cold-hardy varieties in the upper Midwest for which many of the basic multiyear grape flavor and wine style data is needed. Non-destructive sampling included a use of polyvinyl fluoride (PVF) chambers temporarily enclosing and concentrating volatiles emitted by a whole cluster of grapes on a vine and a modified 2 mL glass vial for a vacuum-assisted sampling of volatiles from a single grape berry. We used SPME for either sampling in the field or headspace of crushed grapes in the lab and followed with analyses on gas chromatography-mass spectrometry (GC-MS). We have shown that it is feasible to detect volatile organic compounds (VOCs) emitted in-vivo from single grape berries (39 compounds) and whole clusters (44 compounds). Over 110 VOCs were released to headspace from crushed berries. Spatial (vineyard location) and temporal variations in VOC profiles were observed for all four cultivars. However, these changes were not consistent by growing season, by location, within cultivars, or by ripening stage when analyzed by multivariate analyses such as principal component analysis (PCA) and hierarchical cluster analyses (HCA). Research into aroma compounds present in cold-hardy cultivars is essential to the continued growth of the wine industry in cold climates and diversification of agriculture in the upper Midwestern area of the U.S.
Comments
This article is published as Rice, Somchai, Devin L. Maurer, Anne Fennell, Murlidhar Dharmadhikari, and Jacek A. Koziel. "Evaluation of volatile metabolites emitted in-vivo from cold-hardy grapes during ripening using SPME and GC-MS: A proof-of-concept." Molecules 24, no. 3 (2019): 536. DOI: 10.3390/molecules24030536. Posted with permission.