Historically, sulfur (S) application has not been recommended on Iowa soils for corn and soybean production. Soil supply or combination from sources such as manure or precipitation has met crop S needs. However, soil S levels or supply may become depleted with prolonged crop removal, sulfate leaching, low precipitation deposition, and declining soil organic matter. The objective of this study was to determine if corn and soybean yields would respond to S fertilizer rate and material at multiple sites across Iowa soils and climatic conditions.