Historically sulfur (S) application has not been recommended on Iowa soils for corn and soybean production. The soil supply or combination from sources such as manure or precipitation has met crop S needs. However, soil S levels or supply will become depleted with prolonged crop removal, sulfate leaching, low precipitation deposition, and declining soil organic matter. The objective of this study was to determine the responsiveness of corn and soybean to S application (first year and residual second year) and S fertilizer material at multiple sites across Iowa soils and climatic conditions.