Gibberellin biosynthesis by bacteria and its effect on the rhizobia-legume symbiosis
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The Department of Biochemistry, Biophysics, and Molecular Biology was founded to give students an understanding of life principles through the understanding of chemical and physical principles. Among these principles are frontiers of biotechnology such as metabolic networking, the structure of hormones and proteins, genomics, and the like.
History
The Department of Biochemistry and Biophysics was founded in 1959, and was administered by the College of Sciences and Humanities (later, College of Liberal Arts & Sciences). In 1979 it became co-administered by the Department of Agriculture (later, College of Agriculture and Life Sciences). In 1998 its name changed to the Department of Biochemistry, Biophysics, and Molecular Biology.
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1959–present
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- Department of Biochemistry and Biophysics (1959–1998)
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- College of Agriculture and Life Sciences (parent college)
- College of Liberal Arts and Sciences (parent college)
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Abstract
The gibberellin (GA) phytohormones are integral to many aspects of plant growth and
development. Perhaps due to the importance of GA signaling, many plant-associated
microbes, both fungal and bacterial, are also capable of producing GAs, presumably to
manipulate their plant host. The plant and fungal GA biosynthetic pathways have been well
studied and fully characterized, revealing that these organisms have convergently evolved the
ability to produce GA. However, GA biosynthesis in bacteria has remained elusive. Many
rhizobia, the bacterial, nitrogen-fixing symbionts associated with legumes, have been found
to contain a putative GA biosynthetic gene cluster/operon (GA operon). Through
characterization of the genes from this operon, GA biosynthesis in rhizobia has now been
fully elucidated, thereby providing the first GA biosynthetic pathway for bacteria. Analysis
of this pathway revealed not only that bacteria have convergently evolved GA biosynthesis
independently of plants and fungi, but also that some of the key biochemical transformations
are conserved, including mechanisms of the ring contraction reaction that represents a
committed step in GA biosynthesis. It has further been found that some rhizobia contain an
additional gene that is responsible for converting the non-bioactive product of the GA operon
into a common bioactive form, and phylogenetic analyses suggest that this gene has been
obtained independently of the GA operon itself. Ultimately, it appears that rhizobia produce
GA in order to manipulate their host during symbiosis, specifically by increasing the size of
the nodules in which they reside, thereby increasing the number of bacteria within the nodule
that can be released into the soil. Thus, bacteria seem to have acquired GA biosynthesis in
order to gain a selective advantage in their symbiosis with legumes.