Campus Units
Animal Science, Chemistry, Veterinary Diagnostic and Production Animal Medicine
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
10-2017
Journal or Book Title
Antiviral Research
Volume
146
First Page
28
Last Page
35
DOI
10.1016/j.antiviral.2017.08.006
Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) is the etiological agent of PRRS, an economically significant disease of swine worldwide. PRRSV is poorly controlled by the currently available vaccines, and alternative control strategies are needed to help prevent the continual circulation of the virus. Previously, we developed a synthetic route for the natural compound atractylodinol and demonstrated anti-PRRSV activity in vitro. However, the synthetic route was inefficient and the yield was poor. To identify PRRSV inhibitors that could be synthesized easily and cost-effectively, we synthesized a series of atractylodinol analogs and characterized their anti-PRRSV activity in vitro. A furan-substituted bis-enyne subunit was found to be critical for PRRSV inhibition. Six analogs had potent inhibitory activity against PRRSV with 50% inhibition concentration (IC50) of 0.4–1.4 μM and 50% cytotoxic concentration (CC50) of 209–1537 μM in MARC-145 cells. Three of the most promising compounds also demonstrated significant antiviral activity and low cytotoxicity in porcine macrophages. Inhibition of PRRSV in MARC-145 cells occurred primarily at a post-entry step during PRRSV replication, between 4 and 12 h post-entry. These results suggest that atractylodinol analogs are promising antiviral candidates that could augment current PRRSV control strategies.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Copyright Owner
Elsevier Ltd.
Copyright Date
2017
Language
en
File Format
application/pdf
Recommended Citation
Evans, Alyssa B.; Dong, Pengfei; Loyd, Hyelee; Zhang, Jianqiang; Kraus, George A.; and Carpenter, Susan, "Identification and characterization of small molecule inhibitors of porcine reproductive and respiratory syndrome virus" (2017). Chemistry Publications. 1001.
https://lib.dr.iastate.edu/chem_pubs/1001
Comments
This is a manuscript of an article published as Evans, Alyssa B., Pengfei Dong, Hyelee Loyd, Jianqiang Zhang, George A. Kraus, and Susan Carpenter. "Identification and characterization of small molecule inhibitors of porcine reproductive and respiratory syndrome virus." Antiviral Research (2017). doi: 10.1016/j.antiviral.2017.08.006. Posted with permission.