Campus Units

Chemistry

Document Type

Article

Publication Version

Submitted Manuscript

Publication Date

6-13-2017

Journal or Book Title

Journal of the American Chemical Society

Volume

139

Issue

26

First Page

8950

Last Page

8959

DOI

10.1021/jacs.7b03288

Abstract

We demonstrate continuous high-throughput selective capture of circulating tumor cells by dielectrophoresis at arrays of wireless electrodes (bipolar electrodes, BPEs). The use of BPEs removes the requirement of ohmic contact to individual array elements, thus enabling otherwise unattainable device formats. Capacitive charging of the electrical double layer at opposing ends of each BPE allows an AC electric field to be transmitted across the entire device. Here, two such designs are described and evaluated. In the first design, BPEs interconnect parallel microchannels. Pockets extruding from either side of the microchannels volumetrically control the number of cells captured at each BPE tip and enhance trapping. High-fidelity single-cell capture was achieved when the pocket dimensions were matched to those of the cells. A second, open design allows many non-targeted cells to pass through. These devices enable high-throughput capture of rare cells and single-cell analysis.

Comments

This article is published as Li, Min, and Robbyn K. Anand. "High-throughput selective capture of single circulating tumor cells by dielectrophoresis at a wireless electrode array." Journal of the American Chemical Society (2017). 10.1021/jacs.7b03288. Posted with permission.

Copyright Owner

American Chemical Society

Language

en

File Format

application/pdf

Available for download on Wednesday, June 13, 2018

Published Version

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