Campus Units
Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of, Chemistry
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
11-2017
Journal or Book Title
Plant Physiology
Volume
175
Issue
3
First Page
00652
DOI
10.1104/pp.17.00652
Abstract
Germination is a highly complex process by which seeds begin to develop and establish themselves as viable organisms. In this paper, we utilize a combination of GC-MS, LC-fluorescence, and mass spectrometry imaging (MSI) approaches to profile and visualize the metabolic distributions of germinating seeds from two different inbreds of maize seeds, B73 and Mo17. GC and LC analyses demonstrate that the two inbreds are highly differentiated in their metabolite profiles throughout the course of germination, especially with regard to amino acids, sugar alcohols, and small organic acids. Crude dissection of the seed followed by GC-MS analysis of polar metabolites also revealed that many compounds were highly sequestered among the various seed tissue types. To further localize compounds, matrix-assisted laser desorption/ionization MSI is utilized to visualize compounds in fine detail in their native environments over the course of germination. Most notably, the fatty acyl chain-dependent differential localization of phospholipids and TAGs were observed within the embryo and radicle, showing correlation with the heterogeneous distribution of fatty acids. Other interesting observations include unusual localization of ceramides on the endosperm/scutellum boundary, and subcellular localization of ferulate in the aleurone.
Copyright Owner
American Society of Plant Biologists
Copyright Date
2017
Language
en
File Format
application/pdf
Recommended Citation
Anand, Robbyn; Feenstra, Adam D.; Alexander, Liza E.; Song, Zhihong; Korte, Andrew R.; Yandeau-Nelson, Marna D.; Nikolau, Basil J.; and Lee, Young-Jin, "Spatial Mapping and Profiling of Metabolite Distributions During Germination" (2017). Chemistry Publications. 1016.
https://lib.dr.iastate.edu/chem_pubs/1016
Included in
Molecular Biology Commons, Organic Chemistry Commons, Plant Biology Commons, Plant Breeding and Genetics Commons
Comments
This article is published as Feenstra, Adam D., Liza E. Alexander, Zhihong Song, Andrew R. Korte, Marna Yandeau-Nelson, Basil J. Nikolau, and Young-Jin Lee. "Spatial mapping and profiling of metabolite distributions during germination." Plant Physiology (2017): 00652. 10.1104/pp.17.00652. Posted with permission.