Campus Units

Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of, Chemistry, Genetics, Development and Cell Biology, Ames Laboratory

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

8-2017

Journal or Book Title

Plant Physiology

Volume

174

Issue

4

First Page

2532

Last Page

2548

DOI

10.1104/pp.17.00652

Abstract

Germination is a highly complex process by which seeds begin to develop and establish themselves as viable organisms. In this paper, we utilize a combination of GC-MS, LC-fluorescence, and mass spectrometry imaging (MSI) approaches to profile and visualize the metabolic distributions of germinating seeds from two different inbreds of maize seeds, B73 and Mo17. GC and LC analyses demonstrate that the two inbreds are highly differentiated in their metabolite profiles throughout the course of germination, especially with regard to amino acids, sugar alcohols, and small organic acids. Crude dissection of the seed followed by GC-MS analysis of polar metabolites also revealed that many compounds were highly sequestered among the various seed tissue types. To further localize compounds, matrix-assisted laser desorption/ionization MSI is utilized to visualize compounds in fine detail in their native environments over the course of germination. Most notably, the fatty acyl chain-dependent differential localization of phospholipids and TAGs were observed within the embryo and radicle, showing correlation with the heterogeneous distribution of fatty acids. Other interesting observations include unusual localization of ceramides on the endosperm/scutellum boundary, and subcellular localization of ferulate in the aleurone.

Comments

This article is published as Feenstra, Adam D., Liza E. Alexander, Zhihong Song, Andrew R. Korte, Marna Yandeau-Nelson, Basil J. Nikolau, and Young-Jin Lee. "Spatial mapping and profiling of metabolite distributions during germination." Plant Physiology (2017): 2532. DOI: 10.1104/pp.17.00652 Posted with permission.

Copyright Owner

American Society of Plant Biologists

Language

en

File Format

application/pdf

Published Version

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