Campus Units

Chemistry, Mechanical Engineering, Electrical and Computer Engineering, Plant Sciences Institute

Document Type

Article

Publication Version

Published Version

Publication Date

4-23-2019

Journal or Book Title

Micromachines

Volume

10

Issue

4

First Page

271

DOI

10.3390/mi10040271

Abstract

Clusters of biological cells play an important role in normal and disease states, such as in the release of insulin from pancreatic islets and in the enhanced spread of cancer by clusters of circulating tumor cells. We report a method to pattern cells into clusters having sizes correlated to the dimensions of each electrode in an array of wireless bipolar electrodes (BPEs). The cells are captured by dielectrophoresis (DEP), which confers selectivity, and patterns cells without the need for physical barriers or adhesive interactions that can alter cell function. Our findings demonstrate that this approach readily achieves fine control of cell cluster size over a broader range set by other experimental parameters. These parameters include the magnitude of the voltage applied externally to drive capture at the BPE array, the rate of fluid flow, and the time allowed for DEP-based cell capture. Therefore, the reported method is anticipated to allow the influence of cluster size on cell function to be more fully investigated.

Comments

This article is published as Banovetz, Joseph T., Min Li, Darshna Pagariya, Sungu Kim, Baskar Ganapathysubramanian, and Robbyn K. Anand. "Defining Cell Cluster Size by Dielectrophoretic Capture at an Array of Wireless Electrodes of Several Distinct Lengths." Micromachines 10, no. 4 (2019): 271. DOI: 10.3390/mi10040271. Posted with permission.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Copyright Owner

The Authors

Language

en

File Format

application/pdf

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