Campus Units

Chemistry, Animal Science, Biochemistry, Biophysics and Molecular Biology, Roy J. Carver Department of, Food Science and Human Nutrition, Statistics, Nutritional Sciences, Ames Laboratory

Document Type


Publication Version

Published Version

Publication Date


Journal or Book Title

British Journal of Nutrition





First Page


Last Page





The objective of the present study was to determine whether a mitochondria-targeted vitamin E derivative (MitoVit E) would affect certain mitochondrial parameters, as well as systemic oxidative stress. A total of sixty-four mice were fed a high-fat (HF) diet for 5 weeks. They were then switched to either a low-fat (LF) or a medium-fat (MF) diet, and administered orally with MitoVit E (40 mg MitoVit E/kg body weight) or drug vehicle (10 % (v/v) ethanol in 0·9 % (w/v) NaCl solution), every other day for 5 weeks. Mitochondrial ATP and H2O2 production rates in both the liver and the gastrocnemius were not affected by MitoVit E administration in either LF or MF diet-fed mice. However, the number and average size of the subsarcolemmal mitochondria, but not the intermyofibrillar mitochondria, from the soleus muscle were significantly higher in the MF group receiving MitoVit E (MF-E) than in the MF group receiving vehicle only (MF-C). After the mice were switched from the HF diet to the four dietary treatments (LF-C, LF-E, MF-C and MF-E), the decrease in urinary isoprostane concentration was significantly greater in the LF-E group than in the other three groups during the whole study (weeks 6–10). In addition, MitoVit E significantly increased plasma superoxide dismutase (SOD) activity in the MF diet-fed group without affecting plasma glutathione peroxidase activity or H2O2 levels. Overall, these data suggest that MitoVit E affects subsarcolemmal mitochondrial density and systemic oxidative stress parameters such as plasma SOD activity and urinary isoprostane concentration.


This article is published as Mao, Gaowei, George A. Kraus, Ikyon Kim, Michael E. Spurlock, Theodore B. Bailey, and Donald C. Beitz. "Effect of a mitochondria-targeted vitamin E derivative on mitochondrial alteration and systemic oxidative stress in mice." British Journal of Nutrition 106, no. 1 (2011): 87-95. DOI: 10.1017/S0007114510005830. Posted with permission.

Copyright Owner

The Authors



File Format