Campus Units
Chemistry, Ames Laboratory
Document Type
Article
Publication Version
Accepted Manuscript
Publication Date
5-5-2020
Journal or Book Title
ACS Omega
DOI
10.1021/acsomega.0c01098
Abstract
Ribonucleic acid (RNA) is particularly sensitive to enzymatic degradation by endonucleases prior to sample analysis. In-field preservation has been a challenge for RNA sample preparation. Very recently, hydrophobic magnetic ionic liquids (MIL) have shown significant promise in the area of RNA extraction. In this study, MILs were synthesized and employed as solvents for the extraction and preservation of RNA in aqueous solution. RNA samples obtained from yeast cells were extracted and preserved by the trihexyl(tetradecyl) phosphonium tris(hexafluoroacetylaceto)cobaltate(II) ([P66614+][Co(hfacac)3–]) and trihexyl(tetradecyl) phosphonium tris(phenyltrifluoroacetylaceto)cobaltate(II) ([P66614+][Co(Phtfacac)3–]) MIL with a dispersion of the supporting media, polypropylene glycol, at room temperature for up to a 7 and 15 day period, respectively. High-quality RNA treated with ribonuclease A (RNase A) was recovered from the tetra(1-octylimidazole)cobaltate(II) di(l-glutamate) ([Co(OIM)42+][Glu–]2) and tetra(1-octylimidazole)cobaltate(II) di(l-aspartate) ([Co(OIM)42+][Asp–]2) MILs after a 24 h period at room temperature. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and agarose gel electrophoresis were used to determine the effect of RNA preservation. Furthermore, the preservation mechanism was investigated by exploring the partitioning of RNase A into the MIL using high-performance liquid chromatography.
Copyright Owner
American Chemical Society
Copyright Date
2020
Language
en
File Format
application/pdf
Recommended Citation
Zhu, Chenghui; Varona, Marcelino; and Anderson, Jared L., "Magnetic Ionic Liquids as Solvents for RNA Extraction and Preservation" (2020). Chemistry Publications. 1235.
https://lib.dr.iastate.edu/chem_pubs/1235
Comments
This document is the Accepted Manuscript version of a Published Work that will appear in final form in ACS Omega, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see DOI: 10.1021/acsomega.0c01098. Posted with permission.