Campus Units

Chemistry, Ames Laboratory

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

7-21-2020

Journal or Book Title

Trends in Analytical Chemistry

First Page

115985

DOI

10.1016/j.trac.2020.115985

Abstract

The extraction of nucleic acids (NA) is an essential component of NA analysis. Without sufficient extraction and purification, contaminants co-existing with NAs often inhibit enzymatic amplification causing poor reproducibility and sensitivity. Numerous advancements in NA sampling in recent years have led to improvements in extraction yields from complex matrices and the reduction of analysis times. Perhaps most notable is the push towards miniaturization and automation to facilitate point-of-care testing. This article reviews the advancements and current trends in NA sample preparation including cell lysis and NA extraction.

Comments

This is a manuscript of an article published as Emaus, Miranda N., Marcelino Varona, Derek R. Eitzmann, Shu-An Hsieh, Victoria R. Zeger, and Jared L. Anderson. "Nucleic Acid Extraction: Fundamentals of Sample Preparation Methodologies, Current Advancements, and Future Endeavors." Trends in Analytical Chemistry (2020): 115985. DOI: 10.1016/j.trac.2020.115985. Posted with permission.

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

Copyright Owner

Elsevier B.V.

Language

en

File Format

application/pdf

Available for download on Thursday, July 21, 2022

Published Version

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