Campus Units
Chemistry, Ames Laboratory
Document Type
Article
Publication Version
Published Version
Publication Date
1-28-2021
Journal or Book Title
ACS Omega
DOI
10.1021/acsomega.0c06093
Abstract
Detection of mutations and single-nucleotide polymorphisms is highly important for diagnostic applications. Loop-mediated isothermal amplification (LAMP) is a powerful technique for the rapid and sensitive detection of nucleic acids. However, LAMP traditionally does not possess the ability to resolve single-nucleotide differences within the target sequence. Because of its speed and isothermal nature, LAMP is ideally suited for point-of-care applications in resource-limited settings. Recently, different approaches have been developed and applied to enable single-nucleotide differentiation within target sequences. This Mini-Review highlights advancements in mutation detection using LAMP. Methods involving primer design and modification to enable sequence differentiation are discussed. In addition, the development of probe-based detection methods for mutation detection are also covered.
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.
Copyright Owner
The Authors
Copyright Date
2021
Language
en
File Format
application/pdf
Recommended Citation
Varona, Marcelino and Anderson, Jared L., "Advances in Mutation Detection Using Loop-Mediated Isothermal Amplification" (2021). Chemistry Publications. 1290.
https://lib.dr.iastate.edu/chem_pubs/1290
Included in
Analytical Chemistry Commons, Diagnosis Commons, Nucleic Acids, Nucleotides, and Nucleosides Commons
Comments
This article is published as Varona, Marcelino, and Jared L. Anderson. "Advances in Mutation Detection Using Loop-Mediated Isothermal Amplification." ACS Omega (2021). DOI: 10.1021/acsomega.0c06093. Posted with permission.