Campus Units

Chemistry, Ames Laboratory

Document Type

Article

Publication Version

Published Version

Publication Date

3-29-2012

Journal or Book Title

Journal of the American Chemical Society

Volume

134

Issue

14

First Page

6108

Last Page

6111

DOI

10.1021/ja301332t

Abstract

Novel non-blinking quantum dots (NBQDs) were utilized in three-dimensional super-localization, high-precision tracking applications under an automated scanning-angle total internal reflection fluorescence microscope (SA-TIRFM). NBQDs were randomly attached to stationary microtubules along the radial axis under gliding assay conditions. By automatically scanning through a wide range of incident angles with different evanescent-field layer thicknesses, the fluorescence intensity decay curves were obtained. Fit with theoretical decay functions, the absolute vertical positions were determined with sub-10-nm localization precision. The emission intensity profile of the NBQDs attached to kinesin-propelled microtubules was used to resolve the self-rotation of gliding microtubules within a small vertical distance of ~50 nm. We demonstrate the applicability of NBQDs in high-precision fluorescence imaging experiments.

Comments

Reprinted (adapted) with permission from Journal of the American Chemical Society 134 (2012): 6108, doi:10.1021/ja301332t. Copyright 2012 American Chemical Society.

Copyright Owner

American Chemical society

Language

en

File Format

application/pdf

Share

COinS