Chemistry, Ames Laboratory
Journal or Book Title
Journal of the American Chemical Society
Novel non-blinking quantum dots (NBQDs) were utilized in three-dimensional super-localization, high-precision tracking applications under an automated scanning-angle total internal reflection fluorescence microscope (SA-TIRFM). NBQDs were randomly attached to stationary microtubules along the radial axis under gliding assay conditions. By automatically scanning through a wide range of incident angles with different evanescent-field layer thicknesses, the fluorescence intensity decay curves were obtained. Fit with theoretical decay functions, the absolute vertical positions were determined with sub-10-nm localization precision. The emission intensity profile of the NBQDs attached to kinesin-propelled microtubules was used to resolve the self-rotation of gliding microtubules within a small vertical distance of ~50 nm. We demonstrate the applicability of NBQDs in high-precision fluorescence imaging experiments.
American Chemical society
Marchuk, Kyle; Guo, Yijun; Sun, Wei; Vela, Javier; and Fang, Ning, "High-Precision Tracking with Non-blinking Quantum Dots Resolves Nanoscale Vertical Displacement" (2012). Chemistry Publications. 134.