Campus Units
Chemistry
Document Type
Article
Publication Version
Published Version
Publication Date
11-2012
Journal or Book Title
Langmuir
Volume
48
Issue
49
First Page
17071
Last Page
17078
DOI
10.1021/la303661p
Abstract
Macrocycles made of cholate building blocks were previously found to transport glucose readily across lipid bilayers. In this study, an 15N, 13Cα-labeled glycine was inserted into a cyclic cholate trimer and attached at the end of a linear trimer, respectively. The isotopic labeling allowed us to use solid-state NMR spectroscopy to study the dynamics, aggregation, and depth of insertion of these compounds in lipid membranes. The cyclic compound was found to be mostly immobilized in DLPC, POPC/POPG, and POPC/POPG/cholesterol membranes, whereas the linear trimer displayed large-amplitude motion that depended on the membrane thickness and viscosity. 13C-detected 1H spin diffusion experiments revealed the depth of insertion of the compounds in the membranes, as well as their contact with water molecules. The data support a consistent stacking model for the cholate macrocycles in lipid membranes, driven by the hydrophobic interactions of the water molecules in the interior of the macrocycles. The study also shows a strong preference of the linear trimer for the membrane surface, consistent with its lack of transport activity in earlier liposome leakage assays.
Copyright Owner
American Chemical Society
Copyright Date
2012
Language
en
File Format
application/pdf
Recommended Citation
Wang, Tuo; Widanapathirana, Lakmini Sandachaya; and Zhao, Yan, "Aggregation and Dynamics of Oligocholate Transporters in Phospholipid Bilayers Revealed by Solid-State NMR Spectroscopy" (2012). Chemistry Publications. 185.
https://lib.dr.iastate.edu/chem_pubs/185
Comments
Reprinted (adapted) with permission from Langmuir 28 (2012): doi:10.1021/la303661p. Copyright 2012 American Chemical Society.