Campus Units

Chemistry

Document Type

Article

Publication Version

Published Version

Publication Date

8-13-2009

Journal or Book Title

Journal of Physical Chemistry B

Volume

113

Issue

35

First Page

11999

Last Page

12004

DOI

10.1021/jp905139n

Abstract

The coexistence of different excited states with different properties of the same chromophores could have significant consequences for the accurate characterization of solvation dynamics in a heterogeneous environment, such as a protein. The purpose of this work is to study the contributions of the locally excited (LE) and charge-transferred (CT) states of the fluorescent probe molecule 6-propionyl-2-(N,N-dimethylamino)naphthalene (PRODAN) to its solvation dynamics in the heterogeneous environment provided by reverse micelles formed by sodium 1,4-bis-(2-ethylhexyl) sulfosuccinate (AOT)/n-heptane/water. We have found that the LE and CT states of PRODAN solvate on different time scales in reverse micelles (2 and ∼0.4 ns, respectively), consistent with results suggested in the literature, and have concluded that PRODAN’s use as a probe of heterogeneous environments must be used with caution and that, more importantly, the same caution must be exercised with any chromophore capable of emitting from different excited states.

Comments

Reprinted (adapted) with permission from Journal of Physical Chemistry B 113 (2009): 11999, doi: 10.1021/jp905139n. Copyright 2009 American Chemical Society.

Copyright Owner

American Chemical Society

Language

en

File Format

application/pdf

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