Chemistry, Ames Laboratory
Journal or Book Title
Journal of Mass Spectrometry
1,5-diaminonaphthalene (DAN) has previously been reported as an effective matrix for matrix-assisted laser desorption ioni- zation-mass spectrometry of phospholipids. In the current work, we investigate the use of DAN as a matrix for small metab- olite analysis in negative ion mode. DAN was found to provide superior ionization to the compared matrices for MW < ~400 Da; however, 9-aminoacridine (9-AA) was found to be superior for a uridine diphosphate standard (MW 566 Da). DAN was also found to provide a more representative proﬁle of a natural phospholipid mixture than 9-AA. Finally, DAN and 9-AA were applied for imaging of metabolites directly from corn leaf sections. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.
Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted.
Korte, Andrew R. and Lee, Young-Jin, "MALDI-MS analysis and imaging of smallmolecule metabolites with 1,5-diaminonaphthalene (DAN)" (2014). Chemistry Publications. 941.