Title

Transformation of Hypocotyl Explant Tissue Using Ochrobactrum haywardense H1

Degree Type

Creative Component

Semester of Graduation

Fall 2018

Department

Plant Pathology and Microbiology

First Major Professor

Gary Munkvold

Degree(s)

Master of Science (MS)

Major(s)

Seed Technology and Business

Abstract

The implementation of biotechnology has dramatically increased in industrial practice in the development of crop cultivars due to the advantages of using transgenes to confer desirable traits. Agrobacterium tumefaciens–mediated transformation has been used widely for crop genetic engineering. By applying the transformation method outlined in De Block et al., 1989, as well as initiating classic tissue culture comparisons with the canola line NS1822BC and other commercial elite inbreds, we explored the utility of a proprietary strain of Ochrobactrum haywardense H1 as an alternative to Agrobacterium tumafaciens for canola transformation using shoot regeneration frequency and Zs-Yellow to assess efficiency. A method to successfully transfer DNA from Ochrobactrum into the canola genome, without killing the explant source, will allow an effective alternative to Agrobacterium-mediated transfer.

Copyright Owner

Megan Christenson

File Format

application/pdf

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