Electrical engineering is a field of science dealing with designing of different types of models and apparatus. The paper focuses on the procedures and necessary techniques in the development of a Handheld Smartphone Fluorescent Microscope (HSFM). The HSFM may have a variety of engineering applications. However, the main function of the HSFM in relation to this paper is concerned to develop microscopes that can be used as a blue light detector using the underlying concepts of bio-detector. The device has a wide range of applications and it is normally fixed in electronic gadgets such as mobile phones, specifically at the cameras. The convenience of the device when used on mobile phones is based on their small size enhancing portability unlike other bigger versions. In general, microscopes are used to magnify images or objectives, which is one major role of these devices. However, Fluorescent microscopes have a wide range of use beyond just simple magnification of objects. Such types of microscopes play roles such as the photographic capture and recognition of molecules with near similarity in cell physiology. Its ability to distinguish between two close cells is based on the extremely higher resolution compared to other types of microscopes. With the latter application in mind, the technology used to design fluorescent microscope works to integrate electrical engineering with medicine. The paper focuses on different techniques applied in when designing such microscopes. A significant feature aspect of the research process discussed in the paper is the necessary apparatus and procedures used in the manufacture of the gadget. Besides, such projects usually attract some specific amount of capital input. Therefore, there is a need to include the cost of the entire research in the paper. In addition, the paper has incorporated an in-depth report and analysis of findings in a logical manner for easy synthesis by readers. In the data analysis part, the use of accurate data and other visual presentations have been utilized to bring out a clear relationship between textual and visual information. Ultimately, the research paper herein is a proposal of a unique design of fluorescent microscope.