Degree Type

Creative Component

Semester of Graduation

Spring 2021

Department

Biomedical Sciences

First Major Professor

Jonathan Paul M Mochel

Degree(s)

Master of Science (MS)

Major(s)

Biomedical Sciences

Abstract

The use of CRISPR/Cas is a boom in the news regarding curing genetic diseases. It seems science fiction that someone can cut a mutation and paste the same gene without errors. Many animal studies are using this mechanism. In this case, we explore the use of CRISPR/Cas in organoids extracted from dogs. We present the use of Homology Directed Repair (HDR) to create a WT four base deletion, a PGP mutation. Also, humans have the P-gp protein transcribed by the MDR-1 gene. Its mutations affect P-gp activity. Editing this mutation in dogs could better understand how CRISPR/Cas could help eliminate mutations that affect drug-resistance development and possibly apply them in future human phenotypic and drug analysis.

Copyright Owner

Aguilar Meza, Leeann Shelssya

File Format

PDF

Embargo Period (admin only)

4-14-2021

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