Evaluation of conditions where environmental swabs tested for porcine reproductive and respiratory syndrome virus by quantitative real-time RT-PCR may be used to confirm the decontamination of metal surfaces
Semester of Graduation
Veterinary Diagnostic and Production Animal Medicine
First Major Professor
Master of Science (MS)
Veterinary Preventive Medicine
Because contaminated livestock trailers are a significant risk for transmitting viruses between herds, various methods of washing, disinfecting, and thermo-assisted drying and decontamination (TADD) have been evaluated for their effectiveness in inactivating porcine reproductive and respiratory syndrome virus (PRRSV) on contaminated surfaces. Information on when to expect negative qRT-PCR results after adequate trailer sanitation is lacking. The objective of this study was to evaluate whether there are conditions associated with washing-disinfectant-TADD procedures that will consistently produce a negative qRT-PCR result for the purpose of monitoring compliance with trailer sanitation and decontamination protocols for PRRSV on metal surfaces. 144 diamond plate aluminum coupons were spiked with PRRSV or phosphate-buffered saline (PBS) and treated with a designated disinfectant protocol. Disinfectants evaluated included multiple accelerated hydrogen peroxide (AHP) disinfectants and a quaternary ammonium and glutaraldehyde combination disinfectant. Disinfectant was applied for 5 or 60 minutes of contact time at either 20°C or -10°C in a matrix of feces or PBS. All coupons were heated until the surface temperature of the coupon reached 71°C and then held for 10 minutes to simulate TADD under field conditions. Post-treatment swabs for all treatment groups, except negative control groups, were positive by PRRSV qRT-PCR. Under the conditions evaluated in this study, consistently negative qRT-PCR results after treatments were not found. Therefore, for the purpose of monitoring compliance with trailer sanitation and decontamination protocols for PRRSV, alternatives to qRT-PCR should be explored.
Embargo Period (admin only)
Neat, Rita Anne, "Evaluation of conditions where environmental swabs tested for porcine reproductive and respiratory syndrome virus by quantitative real-time RT-PCR may be used to confirm the decontamination of metal surfaces" (2021). Creative Components. 776.
Large or Food Animal and Equine Medicine Commons, Veterinary Infectious Diseases Commons, Veterinary Microbiology and Immunobiology Commons, Veterinary Preventive Medicine, Epidemiology, and Public Health Commons