Degree Type

Creative Component

Semester of Graduation

Fall 2018

Department

Electrical and Computer Engineering

First Major Professor

Meng Lu

Degree(s)

Master of Science (MS)

Major(s)

Electrical Engineering

Abstract

Rapid and accurate determination of antimicrobial resistance (AMR) of pathogenic bacteria is a national priority owing to its vital importance to public health. We designed this project to develop an on-chip sensor system that is capable of characterizing bacterial drug susceptibility rapidly and inexpensively. First, in order to find an efficient polymerase chain reaction (PCR) method to detect the AMR genes, three different types on-chipmicrofluidic PCR systems were devised and fabricated by utilizing electric-heating, laser-heating based static chambers PCR system, and continuous flow PCR system. Second, we integrated DNA microarray and PCR on one chip to measure the expression level of a large number of AMR genes. The integration of PCR and DNA microarray on chip was fabricated by microfluidics technique. Electric-heating based static chambers PCR system consisted of a polydimethylsiloxane (PDMS) reaction chamber, a micro heater a cooler, a thermocouple, and a thermal controller. Electric-heating based continuous flow PCR system consisted of a thermocycler holder, three polyimide heaters, and 20 cycles Teflon tube. Laser-heating based static chambers PCR system consisted of a plasmonic absorber/thermocouple, a 3W laser, a solenoid shutter, a gasket chamber, and an Arduino controller. Each of PCR system was connected with microarray using microfluidic channels to form a whole integrated AMR sensor chip.

Copyright Owner

Zhihao Liao

Creative Commons License

Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 4.0 License.

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application/pdf

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Engineering Commons

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