Campus Units

Electrical and Computer Engineering, Biomedical Sciences, Materials Science and Engineering, Ames Laboratory

Document Type

Article

Publication Version

Published Version

Publication Date

2018

Journal or Book Title

AIP Advances

Volume

8

Issue

5

First Page

056709

DOI

10.1063/1.5007677

Abstract

Transcranial magnetic stimulation (TMS) is a safe and non-invasive treatment for neurological disorders. TMS has been approved as a treatment for major depressive disorders by the US Food and Drug Administration (FDA) in 2008. Due to the phenomenon of electromagnetic induction, a time-varying magnetic field induces an electric field in the conductive tissues in the brain, TMS has the ability to activate neurons in vivo. However, the effects of the magnetic fields on neurons in cell culture have not been investigated adequately. The magnetic fields affect the neurons when the potential across the neuronal membrane exceeds the threshold which in turn causes an action potential. Based on these theories, we investigated the effects of the magnetic fields generated by a monophasic stimulator with a 70 mm double coil on rat dopaminergic neuronal cell lines (N27). The directions of the magnetic fields in each coil of the double coil oppose each other. The effects of changing the direction of the magnetic field on N27 neurons was also investigated. The results of the experiments showed that both of the fields perpendicular to the coil surface promoted the proliferation of N27 dopaminergic neurons. In order to investigate the gene expression and protein expression affected by TMS, quantitative Polymerase Chain Reaction (qPCR) was used. Here we report changes in glial cell line-derived neurotrophic factor (GDNF) in dopaminergic neuronal cells (N27) after TMS treatment.

Comments

This article is published as Zhong, Xiaojing, Jie Luo, Priyam Rastogi, Anumantha G. Kanthasamy, David C. Jiles, "Transcranial magnetic stimulation promotes the proliferation of dopaminergic neuronal cells in vitro." AIP Advances 8, no. 5 (2018): 056709. DOI: 10.1063/1.5007677. Posted with permission.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Copyright Owner

The Authors

Language

en

File Format

application/pdf

Share

COinS