Campus Units

Electrical and Computer Engineering, Mechanical Engineering, Center for Nondestructive Evaluation (CNDE), Genetics, Development and Cell Biology, Statistics

Document Type


Publication Version

Accepted Manuscript

Publication Date


Journal or Book Title

IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control




There is a critical need to develop new noninvasive therapies to treat bacteria biofilms. Previous studies have demonstrated the effectiveness of cavitation-based ultrasound histotripsy to destroy these biofilms. In this study, the dependence of biofilm destruction on multiple scan parameters was assessed by conducting exposures at different scan speeds (0.3-1.4 beam widths/sec), step sizes (0.25-0.5 beam widths), and number of passes of the focus across the mesh (2-6). For each of the exposure conditions, the number of colony forming units (CFUs) remaining on the mesh was quantified. A regression analysis was then conducted revealing that scan speed was the most critical parameter for biofilm destruction. Reducing the number of passes and the scan speed should allow for more efficient biofilm destruction in the future reducing the treatment time.


This is a manuscript of an article published as Bigelow, T. A., C. L. Thomas, and H. Wu. "Scan Parameter Optimization for Histotripsy Treatment of S. Aureus Biofilms on Surgical Mesh." IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control (2019). DOI: 10.1109/TUFFC.2019.2948305. Posted with permission.


© 2019 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.

Copyright Owner




File Format


Published Version