Document Type

Article

Publication Version

Published Version

Publication Date

6-2014

Journal or Book Title

Journal of the Acoustical Society of America

Volume

135

Issue

6

First Page

3632

Last Page

3638

DOI

10.1121/1.4874627

Abstract

To consider microalgae lipid biofuel as a viable energy source, it is a necessity to maximize algal cell lysis, lipid harvest, and thus biofuel production versus the energy used to lyse the cells. Previous techniques have been to use energy consumptive ultrasound waves in the 10-40 kHz range in a stationary exposure environment. This study evaluated the potential of using 1.1 MHz ultrasound pulses in a new flow through type chamber on Chlamydomonas reinhardtii as a model organism for cell breakage. The ultrasound was generated using a spherically focused transducer with a focal length of 6.34 cm and an active diameter of 6.36 cm driven by 20 cycle sine-wave tone bursts at varied pulse repetition frequencies. First, variations in flow rate were examined at a constant duty cycle of 3.6%. After assessing flow rates, the duty cycle was varied to further explore the dependence on the tone burst parameters. Cell lysis was assessed by quantifying protein and chlorophyll release into the supernatant as well as by lipid extractability. Appropriate flow rates with higher duty cycles led to statistically significant increases in cell lysis relative to controls and other exposure conditions.

Comments

The following article appeared in Journal of the Acoustical Society of America 135 (2014): 3632 and may be found at doi: 10.1121/1.4874627.

Rights

Copyright 2014 Acoustical Society of America. This article may be downloaded for personal use only. Any other use requires prior permission of the author and the Acoustical Society of America.

Copyright Owner

Acoustical Society of America

Language

en

File Format

application/pdf

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