Campus Units

Biochemistry, Biophysics and Molecular Biology, Genetics, Development and Cell Biology, Bioinformatics and Computational Biology

Document Type

Article

Publication Version

Published Version

Publication Date

3-16-2012

Journal or Book Title

BMC Systems Biology

Volume

6

First Page

19

DOI

10.1186/1752-0509-6-19

Abstract

Background
Network motifs, recurring subnetwork patterns, provide significant insight into the biological networks which are believed to govern cellular processes.

Methods
We present a comparative network motif experimental approach, which helps to explain complex biological phenomena and increases the understanding of biological functions at the molecular level by exploring evolutionary design principles of network motifs.

Results
Using this framework to analyze the SM (Sec1/Munc18)-SNARE (N-ethylmaleimide-sensitive factor activating protein receptor) system in exocytic membrane fusion in yeast and neurons, we find that the SM-SNARE network motifs of yeast and neurons show distinct dynamical behaviors. We identify the closed binding mode of neuronal SM (Munc18-1) and SNARE (syntaxin-1) as the key factor leading to mechanistic divergence of membrane fusion systems in yeast and neurons. We also predict that it underlies the conflicting observations in SM overexpression experiments. Furthermore, hypothesis-driven lipid mixing assays validated the prediction.

Conclusion
Therefore this study provides a new method to solve the discrepancies and to generalize the functional role of SM proteins.

Comments

This article is from BMC Systems Biology 6 (2012): 19, doi:10.1186/1752-0509-6-19, Posted with permission.

Rights

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Copyright Owner

Xia et al

Language

en

File Format

application/pdf

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