Campus Units

Ecology, Evolution and Organismal Biology, Entomology, Plant Pathology and Microbiology

Document Type

Article

Publication Version

Published Version

Publication Date

2016

Journal or Book Title

Scientific Reports

Volume

6

First Page

22265

DOI

10.1038/srep22265

Abstract

The honey bee (Apis mellifera) is commonly infected by multiple viruses. We developed an experimental system for the study of such mixed viral infections in newly emerged honey bees and in the cell line AmE-711, derived from honey bee embryos. When inoculating a mixture of iflavirids [sacbrood bee virus (SBV), deformed wing virus (DWV)] and dicistrovirids [Israeli acute paralysis virus (IAPV), black queen cell virus (BQCV)] in both live bee and cell culture assays, IAPV replicated to higher levels than other viruses despite the fact that SBV was the major component of the inoculum mixture. When a different virus mix composed mainly of the dicistrovirid Kashmir bee virus (KBV) was tested in cell culture, the outcome was a rapid increase in KBV but not IAPV. We also sequenced the complete genome of an isolate of DWV that covertly infects the AmE-711 cell line, and found that this virus does not prevent IAPV and KBV from accumulating to high levels and causing cytopathic effects. These results indicate that different mechanisms of virus-host interaction affect virus dynamics, including complex virus-virus interactions, superinfections, specific virus saturation limits in cells and virus specialization for different cell types.

Comments

This article is published as Carrillo-Tripp, Jimena, Adam G. Dolezal, Michael J. Goblirsch, W. Allen Miller, Amy L. Toth, and Bryony C. Bonning. "In vivo and in vitro infection dynamics of honey bee viruses." Scientific reports 6 (2016): 22265. doi: 10.1038/srep22265. Posted with permission.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Copyright Owner

Macmillan Publishers Limited

Language

en

File Format

application/pdf

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