Campus Units

Ecology, Evolution and Organismal Biology

Document Type

Article

Publication Version

Published Version

Publication Date

3-2017

Journal or Book Title

Journal of the National Science Foundation of Sri Lanka

Volume

45

Issue

1

First Page

53

Last Page

65

DOI

10.4038/jnsfsr.v45i1.8038

Abstract

Species of the temperate woody bamboo genus Kuruna Attigala, Kathriar. & L.G. Clark (Poaceae: Bambusoideae) distributed in Sri Lanka and southern India, are threatened due to deforestation and habitat fragmentation. The current study focused on the tetraploid woody bamboo Kuruna debilis (Thwaites) Attigala, Kathriar. & L.G. Clark, using twelve variable microsatellite loci to assess the genetic diversity and population structure in six known Sri Lankan populations. Due to the rarity of the species, an exhaustive sampling of accessible plants resulted in a total of only 28 individuals. Nonetheless, the allelic diversity was high at most loci and given the limited distances separating populations (< 65 km apart), they exhibited a fairly high genetic differentiation (FST = 0.113) and strong isolation by distance. Structure, neighbour-joining, and neighbour-net analyses concur in grouping the six K. debilis populations into three genetic clusters consistent with the spatial proximity of the populations: one cluster comprised populations from the Piduruthalagala Mountain and Horton Plains, the second cluster consisted of the population from Adams Peak and the last comprised the populations from the Handapan Ella Plains. Due to multiple indicators of high allelic diversity, the population from the northern Horton Plains (LA124) should be targeted for conservation. Moreover, the population found in Adams Peak (LA159) is also genetically important and critical to the conservation of these species due to its unique genetic diversity. As the first population genetics study of Bambusoideae in Sri Lanka, we anticipate that our results will provide a foundation for future comparative population genetics and conservation studies in the country.

Comments

This article is published as Attigala, Lakshmi, Timothy Gallaher, John Nason, and Lynn G. Clark. "Genetic diversity and population structure of the threatened temperate woody bamboo Kuruna debilis (Poaceae: Bambusoideae: Arundinarieae) from Sri Lanka based on microsatellite analysis." Journal of the National Science Foundation of Sri Lanka 45, no. 1 (2017). doi: 10.4038/jnsfsr.v45i1.8038. Posted with permission.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Copyright Owner

The Authors

Language

en

File Format

application/pdf

Share

COinS