Campus Units

Ecology, Evolution and Organismal Biology

Document Type

Article

Publication Version

Accepted Manuscript

Publication Date

2019

Journal or Book Title

Genome Biology and Evolution

DOI

10.1093/gbe/evy273

Abstract

Cancer is a threat to multicellular organisms, yet the molecular evolution of pathways that prevent the accumulation of genetic damage has been largely unexplored. The p53 network regulates how cells respond to DNA-damaging stressors. While there has been research on the p53 gene and the transcription factors it encodes, we know little about p53 network regulation. In this study, we performed comparative genetic analyses of the p53 network to quantify the number of genes within the network that are rapidly evolving and constrained, and the association between lifespan and the patterns of evolution. Based on our previous published dataset, we used genomes and transcriptomes of 34 sauropsids and 32 mammals to analyze the molecular evolution of 45 genes within the p53 network. We found that genes in the network exhibited evidence of positive selection and divergent molecular evolution in mammals and sauropsids. Specifically, we found more evidence of positive selection in sauropsids than mammals, indicating that sauropsids have different targets of selection. In sauropsids, more genes upstream in the network exhibited positive selection, and this observation is driven by positive selection in squamates, which is consistent with previous work showing rapid divergence and adaptation of metabolic and stress pathways in this group. Finally, we identified a negative correlation between maximum lifespan and the number of genes with evidence of divergent molecular evolution, indicating that species with longer lifespans likely experienced less variation in selection across the network. In summary, our study offers evidence that comparative genomic approaches can provide insights into how molecular networks have evolved across diverse species.

Comments

This is a manuscript of an article published as Passow, Courtney N., Anne M. Bronikowski, Heath Blackmon, Shikha Parsai, Tonia S. Schwartz, and Suzanne E. McGaugh. "Contrasting patterns of rapid molecular evolution within the p53 network across mammal and sauropsid lineages." Genome biology and evolution (2019). doi: 10.1093/gbe/evy273.

Creative Commons License

Creative Commons Attribution 4.0 License
This work is licensed under a Creative Commons Attribution 4.0 License.

Copyright Owner

The Authors

Language

en

File Format

application/pdf

Published Version

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